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Hidden hyperhydricity may be responsible for abnormal development and acclimatization problems of micropropagated olive plantlets: An anatomical leaf study


TitleHidden hyperhydricity may be responsible for abnormal development and acclimatization problems of micropropagated olive plantlets: An anatomical leaf study
Publication TypeBook
Year of Publication2007
AuthorsGrigoriadou K, Eleftheriou EP, Vasilakakis M
Series TitleActa Horticulturae
Volume748
Number of Pages103-106
ISBN Number05677572
Keywordselectron microscopy, In vitro, Light microscopy, Olea europaea, Olea europaea L
Abstract

Micropropagation of olive has not yet been very successful. Shoot proliferation rate is generally low and cultivar dependent; the formation of adventitious roots in many micropropagated olive cultivars is difficult and the percentage of post-transplanting losses high. The use of liquid medium in a novel temporary immersion system has increased proliferation rate of the Greek olive cultivar 'Chondrolia Chalkidikis' (Olea europaea L.), but met with severe hyperhydricity. These problems inhibited the normal microshoot and root development, resulting in lower acclimatization rates. Samples of leaves from plants derived from cuttings grown in the greenhouse, from normal plants grown in vitro showing no symptoms, and from hyperhydricited plants grown in vitro were fixed for study by light and electron microscopy. The typical characteristics of the species were maintained, but differences between tissues cultured in vitro and control were observed. Symptoms of hyperhydricity were similar with those described for other species. Leaves were thicker, with the palisade parenchyma limited to one layer, intercellular spaces were larger, especially in the spongy parenchyma, and they bore increased number of stomata. The most notable characteristic was, however, the presence of a mucus material in the intercellular spaces, mostly in the area of palisade parenchyma. The same material accumulated also towards the lower epidermal cells. These characteristics, occasionally present even when the symptoms of hyperhydricity were not apparent, were considered as the possible reason of abnormal development and low acclimatization rates of olive microplants cultivated in liquid medium.

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-70449365466&partnerID=40&md5=714c2965ba48d8d9f5c3ae138e482b4c